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. 1994 Oct;32(10):2564-8.
doi: 10.1128/jcm.32.10.2564-2568.1994.

Testing antimicrobial susceptibilities of adherent bacteria by a method that incorporates guidelines of the National Committee for Clinical Laboratory Standards

G Domingue  1 , B Ellis, M Dasgupta, J W Costerton
Affiliations

Testing antimicrobial susceptibilities of adherent bacteria by a method that incorporates guidelines of the National Committee for Clinical Laboratory Standards

G Domingue et al. J Clin Microbiol. 1994 Oct.

Abstract

Membrane filters (0.22-microns pore size) were colonized with 10(4) CFU of logarithmic-phase bacteria per filter under laminar flow conditions in a Modified Robbins Device. Colonized filters were then placed upon agar dilution plates for MIC determinations. Subsequently, filters were transferred to control plates and incubated to obtain MBCs.

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