Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Oct;32(10):2590-2.
doi: 10.1128/jcm.32.10.2590-2592.1994.

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H A Rotbart  1 , M H Sawyer, S Fast, C Lewinski, N Murphy, E F Keyser, J Spadoro, S Y Kao, M Loeffelholz
Affiliations

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay

H A Rotbart et al. J Clin Microbiol. 1994 Oct.

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

PubMed Disclaimer

References

    1. JAMA. 1982 Apr 2;247(13):1843-7 - PubMed
    1. J Infect Dis. 1986 Feb;153(2):346-51 - PubMed
    1. J Clin Microbiol. 1990 Mar;28(3):438-42 - PubMed
    1. J Pediatr. 1990 Jul;117(1 Pt 1):85-9 - PubMed
    1. J Gen Virol. 1990 Sep;71 ( Pt 9):2141-7 - PubMed

LinkOut - more resources