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. 1976 Aug;127(2):988-97.
doi: 10.1128/jb.127.2.988-997.1976.

Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid

M S Guyer, D Figurski, N Davidson

Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid

M S Guyer et al. J Bacteriol. 1976 Aug.

Abstract

pML31, a plasmid chimera constructed to contain the replication genes of an Flac plasmid, has been studied by electron microscope methods. Heteroduplex analysis shows that the only F sequence present in pML31 is that with corrdinates 40.3-49.3F. This region has previously been identified as essential for plasmid maintenance. The sequence of pML31, which was derived originally from R6-5, carries the km gene(s) and an inverted duplication of a 1.0-kilobase sequence. On the basis of length measurements, the repeated sequence is different from IS1, IS2, IS3, and an inverted repeat associated with the km gene(s) of plasmid JR67.

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References

    1. Mol Gen Genet. 1974 May 21;130(2):153-63 - PubMed
    1. J Bacteriol. 1973 May;114(2):577-91 - PubMed
    1. Annu Rev Biochem. 1975;44:45-78 - PubMed
    1. Annu Rev Biochem. 1975;44:273-93 - PubMed
    1. Proc Natl Acad Sci U S A. 1974 Jan;71(1):135-9 - PubMed

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