Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1995 Jan 17;92(2):631-5.
doi: 10.1073/pnas.92.2.631.

Induction of antibodies to the human immunodeficiency virus type 1 by immunization of baboons with immunoglobulin molecules carrying the principal neutralizing determinant of the envelope protein

H Zaghouani  1 , S A Anderson, K E Sperber, C Daian, R C Kennedy, L Mayer, C A Bona
Affiliations

Induction of antibodies to the human immunodeficiency virus type 1 by immunization of baboons with immunoglobulin molecules carrying the principal neutralizing determinant of the envelope protein

H Zaghouani et al. Proc Natl Acad Sci U S A. .

Abstract

The hypervariable region 3 (V3) within the disulfide-bridged loop of the envelope protein of the human immunodeficiency virus type 1 (HIV-1) contains an amino acid sequence that was defined as a principal neutralizing determinant (PND). A 19-amino acid residue consensus sequence (designated V3C) predicted from the PND sequences of 245 isolates as well as a sequence from the PND of the WMJ2 HIV-1 isolate (designated V3M) were expressed on the variable region of murine-human immunoglobulin (Ig) chimeras that were designated Ig-V3C and Ig-V3M, respectively. The HIV-1 sequences on the Ig chimeras preserved their antigenicity and interacted with antibodies specific for peptides encompassing the V3C and V3M sequences. In baboons, Ig-V3C and Ig-V3M induced antibodies that bound V3C and V3M peptides as well as the glycoprotein gp120 envelope protein of HIV-1 MN isolate. In addition, the baboons' antisera were able to prevent infection of CD4 SupT1 susceptible T cells by HIV-1 MN. Finally, Ig-V3M chimeras were able to stimulate in vitro production of antibodies specific for the HIV-1 envelope-derived peptides by lymphocytes from HIV-1-infected human subjects.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Nature. 1986 Apr 10-16;320(6062):535-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11683-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Sep;83(18):7023-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Feb;84(4):1085-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 May;85(9):3198-202 - PubMed

Publication types

MeSH terms