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. 1994 Dec 30;1196(2):227-30.
doi: 10.1016/0005-2736(94)00218-5.

Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

G Brink  1 , L Schmitt, R Tampé, E Sackmann
Affiliations

Self assembly of covalently anchored phospholipid supported membranes by use of DODA-Suc-NHS-lipids

G Brink et al. Biochim Biophys Acta. .

Abstract

We present a novel preparation method for the self assembly of covalently anchored phospholipid supported membranes. The surface is gold covered by cysteamine. Vesicles containing DMPC and activated DODA-Suc-NHS-lipids assembled on this surface. The whole self-assembly process is monitored conveniently by Near Infrared Surface Plasmon Resonance (NIR-SPR). Comparing the data to those obtained by Ca(2+)-mediated vesicle fusion, confirmed this interpretation.

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