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. 1995 Jan 31;92(3):719-23.
doi: 10.1073/pnas.92.3.719.

X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine

L A Lipscomb  1 , M E Peek, M L Morningstar, S M Verghis, E M Miller, A Rich, J M Essigmann, L D Williams
Affiliations

X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine

L A Lipscomb et al. Proc Natl Acad Sci U S A. .

Abstract

We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The results demonstrate that GO forms Watson-Crick base pairs with the opposite C and that G(O) is in the anti conformation. Structural perturbations induced by C.G(O)anti base pairs are subtle. The structure allows us to identify probable elements by which the DNA repair protein MutM recognizes its substrates. Hydrogen bond donors/acceptors within the major groove are the most likely element. In that groove, the pattern of hydrogen-bond donors/acceptors of C.G(O)anti is unique. Additional structural analysis indicates that conversion of G to G(O) would not significantly influence the glycosidic torsion preference of the nucleoside. There is no steric interaction of the 8-oxygen of G(O) with the phospho-deoxyribose backbone.

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References

    1. Nucleic Acids Res. 1992 Nov 25;20(22):6023-32 - PubMed
    1. Chem Biol Interact. 1991;80(3):239-60 - PubMed
    1. J Bacteriol. 1992 Oct;174(20):6321-5 - PubMed
    1. Int J Radiat Biol. 1990 Sep;58(3):397-415 - PubMed
    1. Can J Microbiol. 1993 Sep;39(9):892-4 - PubMed

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