Comparative Study
[Determination of hyaluronidase activity by the rivanol clot method]
[Article in
Russian]
- PMID: 7850237
Item in Clipboard
Comparative Study
[Determination of hyaluronidase activity by the rivanol clot method]
[Article in
Russian]
Klin Lab Diagn.
1994 Sep-Oct.
Abstract
A method for hyaluronidase activity measurement in various biologic samples is suggested, based on rivanol capacity to form a clot with hyaluronic acid inversely proportional to this acid depolymerization under the effect of hyaluronidase. This method sensitivity is ten times higher than that of the mucin clot method; any colorimetric or fluorometric method can be used for detection. The method has been adapted to measurements of commercial hyaluronidase preparations and of blood serum hyaluronidase activity.
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