doi: 10.1007/BF00268089.
The construction in vitro of transducing derivatives of phage lambda
- PMID: 785220
- DOI: 10.1007/BF00268089
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The construction in vitro of transducing derivatives of phage lambda
Mol Gen Genet.
.
Abstract
Methods are described for the construction of plaque-forming, transducing derivatives of phage lambda, using appropirate receptor genomes and fragments of DNA generated by the restriction enzymes endo R.EcoRI and endo R.HindIII. The general properties of the transducing derivatives are described and discussed. Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.
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