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. 1976 Aug 10;147(1):103-9.
doi: 10.1007/BF00337943.

Insertion sequence IS2 near the gene for prophage lambda excision

E Mosharrafa, W Pilacinski, J Zissler, M Fiandt, W Szybalski

Insertion sequence IS2 near the gene for prophage lambda excision

E Mosharrafa et al. Mol Gen Genet. .

Abstract

In this study we characterize a variant of the lambdacI857S7 prophage, designated lambdabi2cI857S7, which carries a DNA insertion. The insertion sequence is IS2, and it resides in the antipolar orientation II just upstream from the gene for prophage excision (xis) at 61.6%lambda. This bi2 insertion mutant could prove valuable for studies on possible recombination functions of IS2 DNA and of its effect on the lambda integration and excision functions.

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