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. 1976 Jun;3(6):1569-76.
doi: 10.1093/nar/3.6.1569.

The separation of ribonucleic acids from Escherichia coli on lysin-agarose

D S Jones, H K Lundgren, F T Jay
Free PMC article

The separation of ribonucleic acids from Escherichia coli on lysin-agarose

D S Jones et al. Nucleic Acids Res. 1976 Jun.
Free PMC article

Abstract

Lysine-agarose provides a simple means of separating RNA species of different moleecular weight. When RNA from Escherichia coli is added to a small column of lysine-agarose and elution is carried out at neutral pH with a shallow linear gradient of NaCl the RNA species are eluted according to size; 4S and 5S RNA species are completely separated and after a delay the 16S and 23S species are eluted as separate peaks. The process is very reproducible and the different species are eluted at a fixed salt concentration even when changes are made in the gradient, provided other conditions, under which the column is run remain constant.

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