doi: 10.1073/pnas.73.8.2569.
In vitro system for induction of delayed early RNA of bacteriophage T4
- PMID: 785471
- PMCID: PMC430689
- DOI: 10.1073/pnas.73.8.2569
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In vitro system for induction of delayed early RNA of bacteriophage T4
Proc Natl Acad Sci U S A.
1976 Aug.
Abstract
Concentrated lysates of Escherichia coli that had been infected with bacteriophage T4 in the presence of chloramphenicol show the same restriction of transcription in vitro as is found in vivo. Restricted lysates can be complemented with lysates from infected cells to induce production of delayed early RNA. Complementation takes place between the RNA polymerase of the restricted lysate and the DNA of the unrestricted lysate. We present evidence that delayed early RNA in these lysates is initiated at quasi-late (middle) promoters, and that such recognition is related to changes in the state of the template DNA.
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