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. 1978 Jul-Aug;35(1-2):10-2.
doi: 10.1111/j.1423-0410.1978.tb02894.x.

A rapid, simple, and reliable technique for preparation of antisera against idiotypes of homogeneous immunoglobulins

J Radl, E de Glopper, G de Groot

A rapid, simple, and reliable technique for preparation of antisera against idiotypes of homogeneous immunoglobulins

J Radl et al. Vox Sang. 1978 Jul-Aug.

Abstract

One-step immunization of guinea pigs with homogeneous immunoglobulins partly purified by micro-agar electrophoresis represents a rapid and reliable microtechnique for the preparation of antisera against the idiotype of the homogeneous immunoglobulins. Testing of these antisera by immunodiffusion techniques requires agar plates containing 3% polyethyleneglycol 6000.

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