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Comparative Study
. 1994 Feb;1(1):1-12.
doi: 10.1007/BF02821507.

The Southern blot. An update

M R Evans  1 , A L Bertera, D W Harris
Affiliations
Comparative Study

The Southern blot. An update

M R Evans et al. Mol Biotechnol. 1994 Feb.

Abstract

This article is devoted to the recent advances made in the Southern blotting technique, which is used for the detection of gel-fractionated DNA molecules following transfer to a membrane. The latest practical improvements made to the techniques of Southern blotting, probe labeling, and hybridization are discussed. Among the most significant advances are: new membranes, transfer methods, probe labeling, and rapid hybridization. Detailed protocols show the application of these improvements in this widely used technique.

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