Lectin affinity chromatography
- PMID: 7866873
- DOI: 10.1007/BF02824807
Lectin affinity chromatography
Abstract
A protocol is described for the preparation of lectin affinity chromatography columns using purified lectins and preactivated matrices. A general method is given for the purification of glycoproteins on immobilized Con A. Methods for immobilizing Con A on CDI agarose, Affi-Gel 15, and carbonyldiimidazole-activated agarose are described.