Comparative Study
doi: 10.1016/0092-8674(95)90517-0.
Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18)
Affiliations
- PMID: 7867070
- DOI: 10.1016/0092-8674(95)90517-0
Item in Clipboard
Comparative Study
Crystal structure of the A domain from the alpha subunit of integrin CR3 (CD11b/CD18)
Cell.
.
Free article
Abstract
We have determined the high resolution crystal structure of the A domain from the alpha chain of integrin CR3. The domain adopts a classic alpha/beta "Rossmann" fold and contains an unusual Mg2+ coordination site at its surface. One of the coordinating ligands is the glutamate side chain from another A domain molecule. We suggest that this site represents a general metal ion-dependent adhesion site (MIDAS) for binding protein ligands. We further propose that the beta subunits of integrins contain a MIDAS motif within a modified A domain. Our crystal structure will allow reliable models to be built for other members of the A domain superfamily and should facilitate development of novel adhesion modulatory drugs.
Similar articles
-
Identification of the binding site for fibrinogen recognition peptide gamma 383-395 within the alpha(M)I-domain of integrin alpha(M)beta2.J Biol Chem. 2001 Apr 27;276(17):13995-4003. doi: 10.1074/jbc.M010174200. Epub 2001 Jan 19. J Biol Chem. 2001. PMID: 11278633
-
Sequence gamma 377-395(P2), but not gamma 190-202(P1), is the binding site for the alpha MI-domain of integrin alpha M beta 2 in the gamma C-domain of fibrinogen.Biochemistry. 2003 Aug 12;42(31):9365-73. doi: 10.1021/bi034057k. Biochemistry. 2003. PMID: 12899623
-
Integrin-ligand binding. Do integrins use a 'MIDAS touch' to grasp an Asp?Curr Biol. 1995 Jun 1;5(6):615-7. doi: 10.1016/s0960-9822(95)00124-2. Curr Biol. 1995. PMID: 7552170 Review.
-
Differential ligand binding specificities of recombinant CD11b/CD18 integrin I-domain.J Biol Chem. 1994 Jun 24;269(25):17075-9. J Biol Chem. 1994. PMID: 7516332
-
Ligand recognition by the I domain-containing integrins.Cell Mol Life Sci. 1998 Jun;54(6):556-66. doi: 10.1007/s000180050184. Cell Mol Life Sci. 1998. PMID: 9676575 Free PMC article. Review.
Cited by
-
Observing force-regulated conformational changes and ligand dissociation from a single integrin on cells.J Cell Biol. 2012 Oct 29;199(3):497-512. doi: 10.1083/jcb.201201091. J Cell Biol. 2012. PMID: 23109670 Free PMC article.
-
McaA and McaB control the dynamic positioning of a bacterial magnetic organelle.Nat Commun. 2022 Sep 26;13(1):5652. doi: 10.1038/s41467-022-32914-9. Nat Commun. 2022. PMID: 36163114 Free PMC article.
-
Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system.Genome Res. 2001 Aug;11(8):1365-74. doi: 10.1101/gr.181001. Genome Res. 2001. PMID: 11483577 Free PMC article.
-
Targeting integrin pathways: mechanisms and advances in therapy.Signal Transduct Target Ther. 2023 Jan 2;8(1):1. doi: 10.1038/s41392-022-01259-6. Signal Transduct Target Ther. 2023. PMID: 36588107 Free PMC article. Review.
-
Locking in alternate conformations of the integrin alphaLbeta2 I domain with disulfide bonds reveals functional relationships among integrin domains.Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2393-8. doi: 10.1073/pnas.041618598. Proc Natl Acad Sci U S A. 2001. PMID: 11226250 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
