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. 1976 Jun;13(6):1699-709.
doi: 10.1128/iai.13.6.1699-1709.1976.

Distribution of dengue-2 antigens by electron immunocytochemistry

R D Cardiff, J K Lund

Distribution of dengue-2 antigens by electron immunocytochemistry

R D Cardiff et al. Infect Immun. 1976 Jun.

Abstract

The distribution of dengue-2 antigens was studied in infected monkey kidney cells (LLC MK2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. This procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to the monolayer. Both virion and nonvirion antigens were identified on the plasma membrane with this technique. A diffuse cytoplasmic reaction product was also present. The intensity and distribution of the cytoplasmic reaction product was related to disruption of the plasma membrane.

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References

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