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. 1994 Oct;26(10):1329-33.
doi: 10.1006/jmcc.1994.1151.

Single pass sequencing of a unidirectional human fetal heart cDNA library to discover novel genes of the cardiovascular system

D M Hwang  1 , W S Hwang, C C Liew
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Single pass sequencing of a unidirectional human fetal heart cDNA library to discover novel genes of the cardiovascular system

D M Hwang et al. J Mol Cell Cardiol. 1994 Oct.

Abstract

A human fetal heart cDNA library was constructed in the lambda gt22A expression vector. Polymerase chain reaction (PCR) was used to amplify the cDNA inserts. PCR products were purified and used in cycle sequencing reactions in the presence of a fluorescein-conjugated primer and electrophoresed on a Pharmacia A.L.F. Sequencer. Partial cDNA sequences, or expressed sequence tags (ESTs) were searched against the Genbank and EMBL databases to identify novel genes expressed in the human cardiovascular system.

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