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Comparative Study
. 1976 Sep;4(3):225-6.
doi: 10.1128/jcm.4.3.225-226.1976.

Comparison of the sensitivity of two methods for isolation of Mycoplasma pneumoniae

R B Craven, R P Wenzel, A M Calhoun, J O Hendley, B H Hamory, J M Gwaltney Jr
Comparative Study

Comparison of the sensitivity of two methods for isolation of Mycoplasma pneumoniae

R B Craven et al. J Clin Microbiol. 1976 Sep.

Abstract

Throat swab specimens from 1,494 Marine Corps recruits were inoculated into vials containing diphasic (broth/agar) mycoplasma medium and also onto PPLO agar plates, which were subsequently overlayed with sheep erythrocytes in saline agar. Strains of Mycoplasma pneumoniae were isolated from 89 (6%) of the specimens by one or both of the methods. Eight-one of the 89 (91%) positive specimens were cultured with diphasic medium, whereas only 42 (47%) were cultured with the overlay method (x2 = 7.44, P less than 0.01). The diphasic system for isolation of M. pneumoniae should be the method of choice in clinical and research application.

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