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. 1978 Jul;23(1):7-13.
doi: 10.1016/S0006-3495(78)85428-9.

Hydrodynamic orientation of sperm heads for flow cytometry

P N Dean, D Pinkel, M L Mendelsohn

Hydrodynamic orientation of sperm heads for flow cytometry

P N Dean et al. Biophys J. 1978 Jul.

Abstract

Two specially shaped sample injection tubes orient flat-shaped mature sperm heads in a flow cytometer. Orientation allows control of a photometric difficulty experienced with some flow cytometers in the measurement of fluorescent and scattered light from sperm and other flat cells. Both relative DNA-stain content and a measure of cell flatness can now be determined simultaneously for each cell at the high rates possible with flow instruments.

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