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. 1976 Sep 23;147(3):263-9.
doi: 10.1007/BF00582877.

Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins

W van Alphen, B Lugtenberg, W Berendsen

Heptose-deficient mutants of Escherichia coli K12 deficient in up to three major outer membrane proteins

W van Alphen et al. Mol Gen Genet. .

Abstract

Mutants of Escherichia coli K12, deficient in up to three major outer membrane proteins b, c and d have been constructed. Mutants that lack the lipopolysaccharide sugar heptose are deficient in protein b. All heptose-deficient strains are supersensitive to lysozyme, various antibiotics and detergents. They excrete the periplasmic enzyme ribonuclease I. Mutants deficient in proteins c and/or d have the same sensitivity towards these compounds as the parent strain. Cells of single, double and triple mutants are all rod-shaped. Electrophoretic analysis of cell envelope proteins indicates that in some mutants the protein deficiency is partially compensated for by increased amounts of one or two of the other major outer membrane proteins. Heptose-deficient strains have an increased amount of 2-keto-3-deoxyoctonate.

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References

    1. J Bacteriol. 1970 Nov;104(2):882-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1963 Sep;50:499-506 - PubMed
    1. J Bacteriol. 1974 Feb;117(2):406-16 - PubMed
    1. J Biol Chem. 1972 Jun 25;247(12):3962-72 - PubMed
    1. J Bacteriol. 1974 Feb;117(2):527-43 - PubMed

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