Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Sep 23;147(3):315-23.
doi: 10.1007/BF00582883.

Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4

A E Jacob, J M Cresswell, R W Hedges, J N Coetzee, J E Beringer

Properties of plasmids constructed by the in vitro insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4

A E Jacob et al. Mol Gen Genet. .

Abstract

Plasmids have been constructed by insertion of DNA from Rhizobium leguminosarum or Proteus mirabilis into RP4 (an R factor of group P). Such recombinant plasmids retain the wide host range of the parental plasmid, being as efficiently transmissible as the unmodified RP4 and are stably maintained in rapidly growing cultures. The recombinant plasmids, even though each contained a DNA sequence absolutely identical with that of the host strain, are no more efficient at mobilizing the transfer of chromosomal genetic information from that host strain than was unmodified RP4. We therefore conclude that an unknown factor must be essential in the process of chromosome mobilization and rate limiting for that process.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 - PubMed
    1. J Gen Microbiol. 1975 Jan;86(1):133-46 - PubMed
    1. J Bacteriol. 1973 Feb;113(2):772-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1959 Jun;45(6):772-85 - PubMed
    1. J Bacteriol. 1971 Dec;108(3):1244-9 - PubMed

LinkOut - more resources