Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Sep;3(9):2387-98.
doi: 10.1093/nar/3.9.2387.

A simple method for DNA restriction site mapping

H O Smith, M L Birnstiel
Free PMC article

A simple method for DNA restriction site mapping

H O Smith et al. Nucleic Acids Res. 1976 Sep.
Free PMC article

Abstract

When a DNA molecule, enzymatically labelled with 32p at one end, is partially digested with a restriction enzyme labelled tdna fragments are obtained which form an overlapping series of molecules, all with a common labelled terminus. ta restriction map can then be constructed from an analysis of the size distribution of these molecules. This technique has been used for the restriction site mapping of cloned histone DNA (h22) where as many as 35 cleavage sites may be accurately determined in a single experiment.

PubMed Disclaimer

References

    1. Biochem Biophys Res Commun. 1974 Jun 4;58(3):586-96 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4 - PubMed
    1. Eur J Biochem. 1976 Jan 2;61(1):101-17 - PubMed
    1. J Mol Biol. 1975 Nov 5;98(3):551-64 - PubMed
    1. Cell. 1976 Aug;8(4):471-8 - PubMed