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. 1994 Aug;38(4):231-3.

Purification of rickettsial cultures contaminated by mycoplasmas

M E Eremeeva  1 , N M Balayeva, D Raoult
Affiliations
  • PMID: 7879714

Purification of rickettsial cultures contaminated by mycoplasmas

M E Eremeeva et al. Acta Virol. 1994 Aug.

Abstract

An experimental biological model is proposed for purification of rickettsiae-infected cell cultures from mycoplasma contamination using intravenous mouse infection and subsequent passages on Vero cell monolayers. Mycoplasma-free rickettsial cultures were obtained in mouse brain or spleen suspension within 3 hrs or 3 days, respectively, after mouse inoculation. Ten strains of spotted fever group rickettsiae were purified from mycoplasmas by this procedure.

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