Serotonin-containing projections to the mesencephalic trigeminal nucleus of the cat

Abstract

Background: It is well known that the mesencephalic trigeminal nucleus (MTN) neurons transmit somatosensory information from proprioceptors in the oral-facial region. Several mechanisms of sensory transduction in these specialized receptors have been proposed, but the neurotransmitters which are responsible for mediating proprioceptive information are still unknown. The current study concentrates on the distribution of one putative neurotransmitter system, serotonin (SER), in the cat MTN. A second objective was to clarify the location and sources of serotoninergic projections on the MTN neurons.

Methods: To determine whether SER was localized in the MTN, the peroxidase-antiperoxidase (PAP) immunocytochemical technique was applied at light and electron microscopic levels in colchicine-treated animals. The origin of SER-containing fibers in the MTN was studied using a double-labeling method combining retrograde transport with wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) and SER immunocytochemistry.

Results: There were no SER-containing neurons in the MTN. The cell bodies of immunonegative MTN neurons were closely surrounded by fine SER-positive fibers and terminals. The labeled fibers were in most cases very thin and sometimes varicose. Ultrastructurally, direct synaptic contacts between SER-containing terminals and perikarya of MTN neurons of all sizes could be seen. The majority of SER-labeled structures were synaptic terminals in which the immunoreactive material was located within the small round clear as well as the small granular vesicles (diameter 50-80 nm) and a few large dense-cored vesicles (up to 150 nm). Retrograde tracing demonstrated that most of cells in the nuclei raphe dorsalis, pontis and magnus were WGA-HRP-labeled.

Conclusions: These results indicated that MTN neurons received serotoninergic projections from the raphe nuclei of the brainstem. In light of these morphological data, it is concluded that the MTN of the cat is under the influence of SER-containing axons and this serotoninergic input may modulate MTN neuronal activity at the first synaptic relay.