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. 1995 Mar;38(3):426-33.
doi: 10.1002/art.1780380321.

Excessive function of peripheral blood neutrophils from patients with Behçet's disease and from HLA-B51 transgenic mice

M Takeno  1 , A Kariyone, N Yamashita, M Takiguchi, Y Mizushima, H Kaneoka, T Sakane
Affiliations

Excessive function of peripheral blood neutrophils from patients with Behçet's disease and from HLA-B51 transgenic mice

M Takeno et al. Arthritis Rheum. 1995 Mar.

Abstract

Objective: To elucidate the role played by HLA-B51 in the neutrophil hyperfunction of Behçet's disease, we determined the superoxide production by purified peripheral blood neutrophils from Behçet's disease patients, from HLA-B51 positive healthy individuals, and from HLA-B51 transgenic mice.

Methods: Neutrophil function was evaluated by flow cytometric analysis, detecting the conversion of 2',7'-dichlorofluorescin diacetate into dichloroflurescein, induced by superoxide in the neutrophils.

Results: A significant correlation between the neutrophil hyperfunction and the possession of HLA-B51 phenotype, regardless of the presence of the disease, was observed in humans. FMLP-stimulated neutrophils (without in vitro priming) from HLA-B51 transgenic mice, but not those from HLA-B35 transgenic mice or from nontransgenic mice, produced substantial amounts of superoxide.

Conclusion: The HLA-B51 molecule itself may be responsible, at least in part, for neutrophil hyperfunction in Behçet's disease.

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