[RNA and protein biosynthesis in toluene treated E. coli B]

Abstract

The conditions for RNA- and protein synthesis in toluenized E. coli B are investigated. The kinetics of RNA- and protein synthesis indicate that a coupled synthesis of both macromolecules takes place in these preparations. Treatment with 1% Brij 58 alters the permeability of toluenized E. coli in such a way that the protein synthesis depends on the addition of supernatant proteins (chromotographed S100 from E. coli) and tRNA.