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. 1995 Jan;61(1):386-8.
doi: 10.1128/aem.61.1.386-388.1995.

Detection of Escherichia coli O157:H7 in meat by an enzyme-linked immunosorbent assay, EHEC-Tek

R P Johnson  1 , R J Durham, S T Johnson, L A MacDonald, S R Jeffrey, B T Butman
Affiliations

Detection of Escherichia coli O157:H7 in meat by an enzyme-linked immunosorbent assay, EHEC-Tek

R P Johnson et al. Appl Environ Microbiol. 1995 Jan.

Abstract

Investigation of the specificity of an enzyme-linked immunosorbent assay (ELISA) for detection of Escherichia coli O157:H7 in raw meats (EHEC-Tek; Organon Teknika Corp.) revealed that the target antigens of the detection reagent, monoclonal antibody 4E8C12, were present in numerous serotypes of E. coli and that their ELISA reactivity was influenced by bile salts, acriflavine, and heat. The specificity of the ELISA was improved by a modified test protocol incorporating immunocapture.

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