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. 1976;13(2):153-9.
doi: 10.1016/0022-1759(76)90153-8.

The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins

S Z Shapiro, T August

The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins

S Z Shapiro et al. J Immunol Methods. 1976.

Abstract

Several factors have been considered in developing an immunoprecipitation procedure which minimizes the background of non-immunospecifically precipitated protein. The procedure evolved utilizes the technique of direct immunoprecipitation (specific antibody plus sufficient unlabeled antigen to form a precipitable complex). High speed centrifugation of the cell extract immediately prior to immunoprecipitation and the use of test tubes coated with silicone greatly reduced background precipitation.

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