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Comparative Study
. 1994 Nov;32(3):336-41.
doi: 10.1006/plas.1994.1074.

Use of firefly luciferase gene for plasmid copy number determination

C Coronado  1 , M E Vázquez, A Cebolla, A J Palomares
Affiliations
Comparative Study

Use of firefly luciferase gene for plasmid copy number determination

C Coronado et al. Plasmid. 1994 Nov.

Abstract

A simple and rapid method for determining plasmid copy number is described. The eukaryotic luc gene is used as a marker to tag plasmid derivatives of several well-known vectors, and by measuring light activity plasmid copy number is determined. A comparative analysis using a standard hybridization procedure to estimate plasmid copy number by densitometry is also described.

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