Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1976 Oct 18;148(2):131-8.
doi: 10.1007/BF00268376.

The molecular mechanism of virus induction. I. A procedure for the biochemical assay of prophage induction

C L Smith, M Oishi

The molecular mechanism of virus induction. I. A procedure for the biochemical assay of prophage induction

C L Smith et al. Mol Gen Genet. .

Abstract

The basic conditions for use of a new biochemical assay of bacteriophage induction are described. This method is based on the read-through transcription of the tryptophan operon integrated into an "early" transcribed region of the bacteriophage phi80 or gamma genome. Inactivation of repressor molecules was assayed by measuring, in the presence of tryptophan, anthranilate synthetase activity in an Escherichia coli (trpE-) phi80 or gamma lysogen infected with phi80ptrp or gammaptrp, respectively, and treated with mitomycin C or UV irradiation. This method provides a sensitive and easy means to analyze the induction process.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3134-8 - PubMed
    1. J Mol Biol. 1967 Jan 28;23(2):247-63 - PubMed
    1. J Bacteriol. 1969 Oct;100(1):231-9 - PubMed
    1. C R Hebd Seances Acad Sci. 1959 Jun 1;248(22):3219-21 - PubMed
    1. Virology. 1967 Feb;31(2):289-95 - PubMed

Publication types

LinkOut - more resources