Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Mar 15;13(6):1434-42.
doi: 10.1002/j.1460-2075.1994.tb06397.x.

Recognition of a DNA operator by a dimer composed of two different homeodomain proteins

C Goutte  1 , A D Johnson
Affiliations

Recognition of a DNA operator by a dimer composed of two different homeodomain proteins

C Goutte et al. EMBO J. .

Abstract

The yeast homeodomain proteins a1 and alpha 2 interact to form a heterodimer that binds DNA with high specificity. The DNA recognition element consists of two similar half sites, arranged with dyad symmetry and separated by a fixed number of base pairs. We demonstrate that in the a1 alpha 2-DNA complex, one of these half-sites is bound by a1 while the other is bound by alpha 2. These assignments allow a comparison of the chemical and nuclease protection patterns produced by both proteins when bound together to the hsg operator. Contrary to simple expectations, we propose that the a1 and alpha 2 homeodomains are arranged on the DNA in tandem, despite the fact that the recognition sequence is dyad symmetric.

PubMed Disclaimer

References

    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. J Mol Biol. 1993 Oct 5;233(3):359-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7860-4 - PubMed
    1. Nature. 1985 Apr 18-24;314(6012):598-603 - PubMed
    1. Cell. 1985 Aug;42(1):237-47 - PubMed

Publication types