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. 1994 May 19;369(6477):207-12.
doi: 10.1038/369207a0.

Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro

S Waga  1 , B Stillman
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Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro

S Waga et al. Nature. .

Abstract

Complete enzymatic replication of DNA from the simian virus 40 origin has been reconstituted with T antigen and highly purified cellular proteins. DNA polymerase-alpha/primase functions primarily to synthesize RNA-DNA primers for initiation of DNA replication at the origin and for priming each Okazaki fragment. A polymerase switching mechanism requiring replication factor C and the proliferating cell nuclear antigen allows two molecules of DNA polymerase-delta to replicate both strands of the double helix conjointly.

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