Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Apr;136(4):1421-34.
doi: 10.1093/genetics/136.4.1421.

RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar

G L Wang  1 , D J Mackill, J M Bonman, S R McCouch, M C Champoux, R J Nelson
Affiliations

RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar

G L Wang et al. Genetics. 1994 Apr.

Abstract

Moroberekan, a japonica rice cultivar with durable resistance to blast disease in Asia, was crossed to the highly susceptible indica cultivar, CO39, and 281 F7 recombinant inbred (RI) lines were produced by single seed descent. The population was evaluated for blast resistance in the greenhouse and the field, and was analyzed with 127 restriction fragment length polymorphism (RFLP) markers. Two dominant loci associated with qualitative resistance to five isolates of the fungus were tentatively named Pi-5(t) and Pi-7(t). They were mapped on chromosomes 4 and 11, respectively. To identify quantitative trait loci (QTLs) affecting partial resistance, RI lines were inoculated with isolate PO6-6 of Pyricularia oryzae in polycyclic tests. Ten chromosomal segments were found to be associated with effects on lesion number (P < 0.0001 and LOD > 6.0). Three of the markers associated with QTLs for partial resistance had been reported to be linked to complete blast resistance in previous studies. QTLs identified in greenhouse tests were good predictors of blast resistance at two field sites. This study illustrates the usefulness of RI lines for mapping a complex trait such as blast resistance and suggests that durable resistance in the traditional variety, Moroberekan, involves a complex of genes associated with both partial and complete resistance.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9981-5 - PubMed
    1. Genetics. 1991 Jan;127(1):181-97 - PubMed
    1. Mamm Genome. 1993;4(6):303-13 - PubMed
    1. Genetics. 1992 Nov;132(3):823-39 - PubMed
    1. Genetics. 1989 Jan;121(1):185-99 - PubMed

Publication types