doi: 10.1016/0304-4165(76)90332-9.
Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock
- PMID: 791366
- DOI: 10.1016/0304-4165(76)90332-9
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Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock
Biochim Biophys Acta.
.
Abstract
Ionic shock treatment in the presence of 10% glycerol is an efficient and selective method for extracting cell surface components from Raji cells and effecting the solubilization of up to 22 polypeptides. The majority of these shock-released polypeptides are accessible to lactoperoxidase radioiodination. Sera from rabbits immunized against these soluble extracts are reactive against Raji cell surface as indicated by indirect membrane immunofluorescence and agglutination assays.
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