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. 1976 Oct 22;444(3):863-74.
doi: 10.1016/0304-4165(76)90332-9.

Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock

E H Lo, M H Ng, W S Ng, S Lui, H C Ho

Study on the surface polypeptides isolated from human lymphoblastoid ce-ls by ionic shock

E H Lo et al. Biochim Biophys Acta. .

Abstract

Ionic shock treatment in the presence of 10% glycerol is an efficient and selective method for extracting cell surface components from Raji cells and effecting the solubilization of up to 22 polypeptides. The majority of these shock-released polypeptides are accessible to lactoperoxidase radioiodination. Sera from rabbits immunized against these soluble extracts are reactive against Raji cell surface as indicated by indirect membrane immunofluorescence and agglutination assays.

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