Cloning and sequencing of a chick embryo cDNA encoding the 20-kDa hypusine-containing protein, eIF-5A
- PMID: 7916728
- DOI: 10.1016/0378-1119(93)90025-x
Cloning and sequencing of a chick embryo cDNA encoding the 20-kDa hypusine-containing protein, eIF-5A
Abstract
Chick embryo contains 18- and 20-kDa isoforms of eukaryotic translation initiation factor 5A (eIF-5A). cDNA clones corresponding to the 20-kDa eIF-5A were isolated and sequenced. A full-length cDNA clone encodes a 153-amino-acid (aa) protein. The deduced aa sequence exactly matches with the partial aa sequence determined for this protein and shows high identity to that of human or rabbit eIF-5A. The results of Southern and Northern hybridization provide evidence for multiple transcripts for chick embryo eIF-5A or an eIF-5A-like protein that presumably derive from more than one gene.
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