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. 1993 Mar;29(3):417-22.

[Design of a chromosomal DNA probe species specific for Yersinia pestis]

[Article in Russian]
O V Norkina, A N Kulichenko, G A Shovadaeva, R Kh Boshnakov, M Iu Aksenov, Iu A Popov, A Ts Tomov, V K K''esovskiĭ, I G Drozdov, A L Gintsburg
  • PMID: 7916733

[Design of a chromosomal DNA probe species specific for Yersinia pestis]

[Article in Russian]
O V Norkina et al. Genetika. 1993 Mar.

Abstract

A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe (designated MK) was constructed on the basis of the T16 fragment. As judged from restriction analysis, blot-hybridization experiments and, partially, sequencing, significant homology exists between the MK DNA probe and this one developed by Bardarov et. al. (1990). A repeated sequence in two copies was discovered in the MK fragment.

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