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. 1976 Nov;97(1):73-82.
doi: 10.1099/00221287-97-1-73.

Location of the Aspartase Gene (aspA) on the linkage map of Escherichia coli K12

M E Spencer, V M Lebeter, J R Guest

Location of the Aspartase Gene (aspA) on the linkage map of Escherichia coli K12

M E Spencer et al. J Gen Microbiol. 1976 Nov.

Abstract

The aspartase (L-asparatate ammonia-lyase, EC. 4.3.I.I) structural gene, aspA, was mapped by two-factor and three-factor transductional crosses using phage PI. Cotransduction frequencies between aspA and other markers were: ampA (69%); frdA (48 TO 67%) mel (35%); purA (17%); fdp (I-6%). The sequence of markers within the corresponding segment (91 to 95 min) of the Escherichia coli linkage map was shown to be mel-aspA-ampA-frdA-purA-fdp.

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