doi: 10.1038/263744a0.
Cloned synthetic lac operator DNA is biologically active
- PMID: 792711
- DOI: 10.1038/263744a0
Item in Clipboard
Cloned synthetic lac operator DNA is biologically active
Nature.
.
Abstract
A chemically synthesised duplex DNA fragment containing the sequence of the lac operator was cloned in E. coli using the vehicle pMB9. Clones containing lac-pMB9 hybrid DNA produced beta-galactosidase constitutively and the hybrid DNA bound the lac repressor specifically.
Similar articles
-
Synthetic lac operator DNA is functional in vivo.Nature. 1976 Oct 28;263(5580):748-52. doi: 10.1038/263748a0. Nature. 1976. PMID: 1069185 No abstract available.
-
Cloned seventeen-nucleotide-long synthetic lactose operator is biologically active.Gene. 1978 Apr;3(2):123-34. doi: 10.1016/0378-1119(78)90056-2. Gene. 1978. PMID: 95988
-
Construction, isolation and implications of repressor-galactosidase - beta-galactosidase hybrid molecules.Eur J Biochem. 1977 Oct 3;79(2):381-6. doi: 10.1111/j.1432-1033.1977.tb11819.x. Eur J Biochem. 1977. PMID: 411650
-
Synthesis and cloning of operator DNA of the lac operon of E. coli.Can J Biochem. 1977 Nov;55(11):1125-33. doi: 10.1139/o77-168. Can J Biochem. 1977. PMID: 336161 Review. No abstract available.
-
Interaction of regulator proteins with recognition sequences of DNA.Cell. 1974 May;2(1):1-7. doi: 10.1016/0092-8674(74)90002-6. Cell. 1974. PMID: 4606676 Review. No abstract available.
Cited by
-
Regulation of the F-factor pif operon: pifO, a site required in cis for autoregulation, titrates the pifC product in trans.J Bacteriol. 1984 Oct;160(1):192-8. doi: 10.1128/jb.160.1.192-198.1984. J Bacteriol. 1984. PMID: 6090412 Free PMC article.
-
Rapid synthesis of oligodeoxyribonucleotides: a new solid-phase method.Nucleic Acids Res. 1977 Apr;4(4):1135-58. doi: 10.1093/nar/4.4.1135. Nucleic Acids Res. 1977. PMID: 866189 Free PMC article.
-
Relationship between messenger ribonucleic acid and enzyme levels specified by the leucine operon of Escherichia coli K-12.J Bacteriol. 1977 Sep;131(3):997-1007. doi: 10.1128/jb.131.3.997-1007.1977. J Bacteriol. 1977. PMID: 330509 Free PMC article.
-
Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene.J Bacteriol. 1982 Feb;149(2):587-94. doi: 10.1128/jb.149.2.587-594.1982. J Bacteriol. 1982. PMID: 6276362 Free PMC article.
-
Molecular cloning of DNA. An introduction into techniques and problems.Hum Genet. 1977 Dec 29;40(1):1-72. doi: 10.1007/BF00280831. Hum Genet. 1977. PMID: 342388 Review. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
