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. 1976 Oct 28;263(5580):744-8.
doi: 10.1038/263744a0.

Cloned synthetic lac operator DNA is biologically active

K J Marians, R Wu, J Stawinski, T Hozumi, S A Narang

Cloned synthetic lac operator DNA is biologically active

K J Marians et al. Nature. .

Abstract

A chemically synthesised duplex DNA fragment containing the sequence of the lac operator was cloned in E. coli using the vehicle pMB9. Clones containing lac-pMB9 hybrid DNA produced beta-galactosidase constitutively and the hybrid DNA bound the lac repressor specifically.

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