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Multicenter Study
. 1994 Jul;32(7):1625-8.
doi: 10.1128/jcm.32.7.1625-1628.1994.

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M A Pfaller  1 , Q Vu, M Lancaster, A Espinel-Ingroff, A Fothergill, C Grant, M R McGinnis, L Pasarell, M G Rinaldi, L Steele-Moore
Affiliations
Multicenter Study

Multisite reproducibility of colorimetric broth microdilution method for antifungal susceptibility testing of yeast isolates

M A Pfaller et al. J Clin Microbiol. 1994 Jul.

Abstract

MICs of fluconazole and amphotericin B were determined independently for 100 coded yeast isolates by each of six laboratories to determine reproducibility of results by using a colorimetric oxidation-reduction-based broth microdilution test. In addition, each site tested five quality control isolates on at least four different occasions during the study. Results agreed within a three-dilution range (mode +/- 1 log2 dilution) for 96.2% of fluconazole tests and 92.7% of amphotericin B tests. Agreement among tests with the quality control isolates was 99.4% with fluconazole and 98.6% with amphotericin B. These results indicate that the colorimetric microdilution method is reproducible among laboratories.

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