[Detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)]
[Article in
German]
- PMID: 79307
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[Detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)]
[Article in
German]
Allerg Immunol (Leipz).
1978.
Abstract
The macrophage adherence inhibition test is a suitable assay for detection of cell-mediated immunity against bovine gamma globulin (BGG) in guinea pigs. The test was performed with peritoneal cells (PC) in hemocytometer chambers. 10 mg/ml BGG were added as antigen. The washing step was performed with a burette. An average standard deviation of 5% was obtained with this washing method. Day-to -day-investigations in individual animals show differences with respect to the degree of cell-mediated immunity against BGG. Supernatants of sensitized spleen cells or lymph node cells produce adherence inhibition in PC of nonimmunized guinea pigs too.
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