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. 1978;24(1):61-9.

[Detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)]

[Article in German]
R Schimke, C Eichler, H Ambrosius
  • PMID: 79307

[Detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)]

[Article in German]
R Schimke et al. Allerg Immunol (Leipz). 1978.

Abstract

The macrophage adherence inhibition test is a suitable assay for detection of cell-mediated immunity against bovine gamma globulin (BGG) in guinea pigs. The test was performed with peritoneal cells (PC) in hemocytometer chambers. 10 mg/ml BGG were added as antigen. The washing step was performed with a burette. An average standard deviation of 5% was obtained with this washing method. Day-to -day-investigations in individual animals show differences with respect to the degree of cell-mediated immunity against BGG. Supernatants of sensitized spleen cells or lymph node cells produce adherence inhibition in PC of nonimmunized guinea pigs too.

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