GABA and glycine have different effects on monaural response properties in the dorsal nucleus of the lateral lemniscus of the mustache bat

Abstract

1. We studied the monaural response properties of 81 neurons in the dorsal nucleus of the lateral lemniscus (DNLL) of the mustache bat before and during the iontophoretic application of antagonists that blocked gamma-aminobutyric acid-A (GABAA) receptors (bicuculline) or glycine receptors (strychnine). The main finding is that GABAergic inhibition had substantial effects, whereas glycine had little or no effect on the activity evoked by contralateral stimulation. 2. Before the application of drugs, the monaural response properties of DNLL cells were characterized by two main features. The first was that the majority (86%) of neurons had monotonic rate-intensity functions, whereas only 14% had weakly nonmonotonic functions. The second was that most (66%) neurons displayed some form of chopping response pattern, in which there was a regular interval between discharges that was unrelated to the period of the tone burst frequency. 3. Bicuculline had two major effects on the majority of DNLL cells. It caused large increases in spike counts and changes in temporal discharge patterns. In 38 of 47 cells (81%) bicuculline changed the temporal discharge patterns into a sustained chopper pattern. In addition, the duration of the discharge train continued for a period of time longer than the duration of the tone burst in many but not all neurons. Prolonged firing of this sort was rarely seen in the predrug condition. Furthermore, in a few cells bicuculline caused a decrease in the interspike interval as well as a lengthening of the discharge train. 4. Blocking glycine, in contrast, caused either small increases in spike count or no increase at all and did not affect the temporal discharge patterns in the majority (87%) of neurons. 5. In most DNLL cells the shapes of the rate-intensity functions were virtually the same before and during the application of either antagonist. The rate-intensity functions of 91% of the cells were unaffected by bicuculline and 98% were unaffected by strychnine. 6. Blocking either GABAergic inhibition or glycinergic inhibition had no effect on discharge latency in the vast majority of DNLL cells. In a few neurons application of bicuculline or strychnine had a small influence and caused discharge latency to decrease by < or = 1 ms. 7. These results show that the excitation from stimulation of the contralateral ear evokes a sustained chopping discharge pattern in the vast majority of DNLL neurons. The sustained chopping response is changed into another discharge pattern by the GABAergic innervation that is also evoked by stimulation of the contralateral ear.(ABSTRACT TRUNCATED AT 400 WORDS)