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. 1994 Nov;68(11):7030-9.
doi: 10.1128/JVI.68.11.7030-7039.1994.

Template-dependent, in vitro replication of rotavirus RNA

D Chen  1 , C Q Zeng, M J Wentz, M Gorziglia, M K Estes, R F Ramig
Affiliations

Template-dependent, in vitro replication of rotavirus RNA

D Chen et al. J Virol. 1994 Nov.

Abstract

A template-dependent, in vitro rotavirus RNA replication system was established. The system initiated and synthesized full-length double-stranded RNAs on rotavirus positive-sense template RNAs. Native rotavirus mRNAs or in vitro transcripts, with bona fide 3' and 5' termini, derived from rotavirus cDNAs functioned as templates. Replicase activity was associated with a subviral particle containing VP1, VP2, and VP3 and was derived from native virions or baculovirus coexpression of rotavirus genes. A cis-acting signal involved in replication was localized within the 26 3'-terminal nucleotides of a reporter template RNA. Various biochemical and biophysical parameters affecting the efficiency of replication were examined to optimize the replication system. A replication system capable of in vitro initiation has not been previously described for Reoviridae.

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References

    1. Virus Res. 1986 Dec;6(3):217-33 - PubMed
    1. J Virol. 1987 May;61(5):1488-94 - PubMed
    1. Virology. 1988 Oct;166(2):358-65 - PubMed
    1. Virology. 1989 Apr;169(2):427-35 - PubMed
    1. EMBO J. 1989 Mar;8(3):947-54 - PubMed

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