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. 1994 Oct 13;371(6498):612-4.
doi: 10.1038/371612a0.

Activation of Raf-1 by 14-3-3 proteins

W J Fantl  1 , A J Muslin, A Kikuchi, J A Martin, A M MacNicol, R W Gross, L T Williams
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Activation of Raf-1 by 14-3-3 proteins

W J Fantl et al. Nature. .

Abstract

The protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood. Using the yeast two-hybrid system, we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA2) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins. Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

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