doi: 10.1016/0304-3940(94)90180-5.
Stable transfection of the NR1 subunit in Chinese hamster ovary cells fails to produce a functional N-methyl-D-aspartate receptor
Affiliations
- PMID: 7936412
- DOI: 10.1016/0304-3940(94)90180-5
Item in Clipboard
Stable transfection of the NR1 subunit in Chinese hamster ovary cells fails to produce a functional N-methyl-D-aspartate receptor
Neurosci Lett.
.
Abstract
The NR1 subunit of the N-methyl-D-aspartate (NMDA) receptor cDNA was stably transfected into Chinese hamster ovary (CHO) cells. Northern analysis revealed 3 clonal cell lines expressing high levels of NMDAR1 mRNA (BA1, BA2 and BA3). NMDAR1 protein was readily detected by Western analysis in only one of the clonal populations, BA1. Whole-cell patch clamp analysis revealed a lack of functional NMDA receptors in all of the cell lines, including BA1. NMDA-mediated responses were obtained only after a second subunit, NMDAR2A, was transiently expressed in BA1 cells. Therefore, the NR1 subunit alone may not be sufficient to form functional NMDA receptors in CHO cells.
Similar articles
-
Pharmacological properties of acquired excitotoxicity in Chinese hamster ovary cells transfected with N-methyl-D-aspartate receptor subunits.J Pharmacol Exp Ther. 1996 Nov;279(2):515-23. J Pharmacol Exp Ther. 1996. PMID: 8930153
-
Inhibition by intracellular Mg(2+) of recombinant N-methyl-D-aspartate receptors expressed in Chinese hamster ovary cells.J Pharmacol Exp Ther. 2000 Mar;292(3):1104-10. J Pharmacol Exp Ther. 2000. PMID: 10688629
-
A critical role of the N-methyl-D-aspartate (NMDA) receptor subunit (NR) 2A in the expression of redox sensitivity of NR1/NR2A recombinant NMDA receptors.J Pharmacol Exp Ther. 1999 Nov;291(2):785-92. J Pharmacol Exp Ther. 1999. PMID: 10525101
-
Effects of acute and chronic ethanol exposure on heteromeric N-methyl-D-aspartate receptors expressed in HEK 293 cells.J Neurochem. 1997 Dec;69(6):2345-54. doi: 10.1046/j.1471-4159.1997.69062345.x. J Neurochem. 1997. PMID: 9375665
-
Effects of amino acid substitutions in transmembrane domains of the NR1 subunit on the ethanol inhibition of recombinant N-methyl-D-aspartate receptors.Alcohol Clin Exp Res. 2006 Mar;30(3):523-30. doi: 10.1111/j.1530-0277.2006.00058.x. Alcohol Clin Exp Res. 2006. PMID: 16499494
Cited by
-
High-fidelity transmission acquired via a developmental decrease in NMDA receptor expression at an auditory synapse.J Neurosci. 2001 May 15;21(10):3342-9. doi: 10.1523/JNEUROSCI.21-10-03342.2001. J Neurosci. 2001. PMID: 11331363 Free PMC article.
-
The Redox Biology of Excitotoxic Processes: The NMDA Receptor, TOPA Quinone, and the Oxidative Liberation of Intracellular Zinc.Front Neurosci. 2020 Jul 24;14:778. doi: 10.3389/fnins.2020.00778. eCollection 2020. Front Neurosci. 2020. PMID: 32792905 Free PMC article. Review.
-
Functional consequences of NR2 subunit composition in single recombinant N-methyl-D-aspartate receptors.Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):11019-24. doi: 10.1073/pnas.94.20.11019. Proc Natl Acad Sci U S A. 1997. PMID: 9380752 Free PMC article.
-
N-methyl-D-aspartate receptor mechanosensitivity is governed by C terminus of NR2B subunit.J Biol Chem. 2012 Feb 3;287(6):4348-59. doi: 10.1074/jbc.M111.253740. Epub 2011 Dec 16. J Biol Chem. 2012. PMID: 22179603 Free PMC article.
-
Brain-specific regulator of G-protein signaling 9-2 selectively interacts with alpha-actinin-2 to regulate calcium-dependent inactivation of NMDA receptors.J Neurosci. 2006 Mar 1;26(9):2522-30. doi: 10.1523/JNEUROSCI.4083-05.2006. J Neurosci. 2006. PMID: 16510730 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
