Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1994 Sep 25;22(19):3928-9.
doi: 10.1093/nar/22.19.3928.

New restriction endonuclease CviRI cleaves DNA at TG/CA sequences

A Jin  1 , Y Zhang, Y Xia, E Traylor, M Nelson, J L Van Etten
Affiliations
Free PMC article

New restriction endonuclease CviRI cleaves DNA at TG/CA sequences

A Jin et al. Nucleic Acids Res. .
Free PMC article

Abstract

A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.

PubMed Disclaimer

References

    1. J Mol Biol. 1968 Jul 14;34(2):373-5 - PubMed
    1. J Clin Invest. 1985 Aug;76(2):894-7 - PubMed
    1. Mol Cell Biol. 1986 May;6(5):1430-9 - PubMed
    1. Hum Genet. 1988 Feb;78(2):151-5 - PubMed
    1. Hum Genet. 1988 Apr;78(4):299-312 - PubMed

Publication types