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Comparative Study
. 1994 Oct 11;22(20):4039-43.
doi: 10.1093/nar/22.20.4039.

5-Nitroindole as an universal base analogue

D Loakes  1 , D M Brown
Affiliations
Free PMC article
Comparative Study

5-Nitroindole as an universal base analogue

D Loakes et al. Nucleic Acids Res. .
Free PMC article

Abstract

4-, 5- and 6-Nitroindole have been investigated and compared with 3-nitropyrrole as universal bases in oligodeoxynucleotides. Of these the 5-nitroindole derivative was found to be superior giving higher duplex stability, and behaving indiscriminately towards each of the four natural bases in duplex melting experiments. 3-Nitropyrrole, whilst not discriminating between the natural bases, was found to lead to considerable destabilisation of the duplexes, particularly when multiple substitutions were made, in contrast to the 5-nitroindole nucleoside.

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References

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