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. 1994 Oct 11;22(20):4132-8.
doi: 10.1093/nar/22.20.4132.

Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor

J Caubín  1 , T Iglesias, J Bernal, A Muñoz, G Márquez, J L Barbero, A Zaballos
Affiliations
Free PMC article

Isolation of genomic DNA fragments corresponding to genes modulated in vivo by a transcription factor

J Caubín et al. Nucleic Acids Res. .
Free PMC article

Abstract

A new methodology for the identification of genes modulated by transcription factors in vivo is described. Mouse genomic DNA fragments bound by the thyroid hormone receptor (T3R) were selected and amplified in vitro. Subsequent hybridisation with biotinylated cDNA allowed the selection of those DNA fragments containing binding sites for T3R that corresponded to transcribed DNA. Expression analysis of the corresponding genes showed that more than 80% are indeed modulated by thyroid hormones in vivo in the liver. Together with the presence of consensus binding sites for T3R this result suggests that the selected DNA fragments may contain T3R transcriptional regulatory elements. This method, extensive to other ligand-modulated transcription factors, might be useful to all transcription factors with slight modifications.

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References

    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. J Biol Chem. 1994 Feb 11;269(6):4175-9 - PubMed
    1. Cell. 1983 Sep;34(2):557-67 - PubMed
    1. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 - PubMed
    1. FEBS Lett. 1984 Feb 27;167(2):263-8 - PubMed

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