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. 1994 Aug;53(8):506-10.
doi: 10.1136/ard.53.8.506.

Increased endothelin-1 production in fibroblasts derived from patients with systemic sclerosis

Y Kawaguchi  1 , K Suzuki, M Hara, T Hidaka, T Ishizuka, M Kawagoe, H Nakamura
Affiliations

Increased endothelin-1 production in fibroblasts derived from patients with systemic sclerosis

Y Kawaguchi et al. Ann Rheum Dis. 1994 Aug.

Abstract

Objectives: To determine whether fibroblasts from patients with systemic sclerosis (SSc) produce excessive amounts of endothelin-1 (ET-1), which is recognised as having vasoconstrictive properties and as having a potent mitogenic effect on fibroblasts.

Methods: Dermal fibroblasts were removed from 11 patients with SSc and from five normal controls (NC). The assay of ET-1 protein was measured by an ELISA that used two anti-ET-1 antibodies. The gene expression of prepro ET-1 mRNA was evaluated by a reverse-transcriptase polymerase chain reaction (RT-PCR) method.

Results: Levels of ET-1 protein were significantly higher in SSc fibroblast cultures than in those of normal fibroblasts (p < 0.01). The expression of prepro ET-1 mRNA was also higher in SSc fibroblasts than in normal fibroblasts. The addition of interleukin-1 beta (IL-1 beta) increased the production of ET-1 by fibroblasts.

Conclusion: The findings indicate that the overproduction of ET-1 is a novel abnormal function in SSc fibroblasts, and that ET-1 induced by fibroblasts may play a role in the fibrosis and Raynaud's phenomenon of SSc.

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References

    1. J Rheumatol. 1992 Aug;19(8):1207-11 - PubMed
    1. Life Sci. 1992;50(4):247-55 - PubMed
    1. J Rheumatol. 1992 Nov;19(11):1710-5 - PubMed
    1. Biochem Biophys Res Commun. 1993 Sep 15;195(2):1050-6 - PubMed
    1. Atherosclerosis. 1993 Jul;101(2):185-90 - PubMed